Live phages and Col-SK-viruses as well as inactivated, radiomarked S. typhi murium bacteria after oral induction are persorbed by the intestine of the mouse and soon appear in the blood. These experiments were extended by three more test models: live staphylococci and S. typhi murium bacteria as well as C. albicans germs. The test organisms are persorbed rather independent on the replication state, the size, and the quantity of the test organisms. The time curves are so similar that there a generally valid norm curve of persorption kinetics could be developed. This curve attains its climax between 30 and 60 min. after induction of the germs and declines to the nadir after 8 hours. If phages are induced by the intravenous route as test model they occur at the same speed in the opposite direction in the gastric contents; the curve of this secretion is very similar to that of the persorption. The courses of persorption are described as Herbst-effect by experiments of Volkheimer and co-workers. According to this, orally induced particles may be persorbed in the body up to a size of 50 microns paracellulary by the mucosal epithelium of the small intestine. In that way the oral vaccination becomes effective.
Orale Immunisierung mit Nichtvermehrungsfaehigen Mikroorganismen oder ihren Antigenen. 13. Mitteilung: Persorption und Sekretion von Mikroorganismen im Tierversuch (Oral Immunization with Non-Living Microorganisms or Their Antigens. 13).